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    Sequence analysis
    Posted by: Shia (IP Hidden, New member, 3)
    Date: November 10, 2005 10:09AM

    Good day.
    This is a VERY simple question (and solution that I do not know).

    I have sequenced an 870 bp fragment of my bacterial genome, (a particular gene).
    I have BLASTed the sequence into GENbank, and I would know like to do analysis such as looking for variable and conserved regions. Possible mutations in the region and general differences.

    Is there software for this? Or is it done by eye?

    Can anyone give me a step by step guide on what to do with sequences once they have been sequenced?
    Thank you

     

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    Re: Sequence analysis
    Posted by: jiangbd (IP Hidden, Unregistered user, )
    Date: November 15, 2005 06:10PM

    You can do sequence similarity analysis using programs from GCG which is a commercial UNIX program package. Or you can find EMBOSS package which is free from the internet. Both software suite have sequence analysis programs with different purposes. You need to read the help file to learn how to use each program and what can they do. Best.

    JiangBD

     

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