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can only detect overexpressed, but not endogenous, protein
Posted by: wbd2975 (IP Hidden, New member, 5)
Date: May 10, 2005 12:13PM
Has anyone out there had the problem of only being able to detect overexpressed protein, but not endogenous levels in cells which should produce (albeit low amounts of) the protein, on Westerns? If so, what manipulations, if any, allowed detection of endogenous protein? I've tried standard troubleshooting (different Ab concentrations, different masses of loaded protein, different lysis buffers, different lysis protocols).
Any ideas would be much appreciated.
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