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Method to freeze hybridoma
Posted by: gurumoorthyk (IP Hidden, New member, 1)
Date: July 28, 2005 08:17AM

Hi,
I am generating hybridomas from transgenic mouse spleen inorder to analyse the repertoire (Light chain). I ended up having more than 300 master clones (and many more to come) from my fusions and i am not able to freeze all of them in conventional way since space is limited. I was wondering is there any freezeing method available in 96 well format or any other method which can occupy less space in liquid nitrogen tank. I would greatly acknowledge if anyone has such technology to do it.

thanks in advance
Guru

 

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