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phosphorylation detection kit?
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: July 26, 2005 09:18AM
Anyone know a kit can be used to detect phosphotyrosin, phosphoserine and phosphothreonine sites? Thanks.
Re: phosphorylation detection kit?
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: July 27, 2005 12:17PM
mitolab, check out Qiagen. they have a kit (LiquiChip Broad Range Kinase kit) that offers non-radioactive quantification and screening of kinase residues (tryosine, serine and threonine) in cell culture and tissue samples. if the kit proves to be too expensive they also offer individual antibodies to screen for serine, tyrosine, or threonine sites. hope this helps. after all, i owe you one. :)
Re: phosphorylation detection kit?
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: July 28, 2005 06:43AM
hi femmeauburn, thanks for the information. I also come up with some other vendors but no one seems offer pannel of antibodies in one kit. Your suggestion is very useful. It seems anti-phosphotyrosine is cheap while others are bloody expensive.
Re: phosphorylation detection kit?
Posted by: dval7780 (IP Hidden, New member, 1)
Date: October 10, 2005 09:57AM
Check out Calbiochem. they have a Phosphodetect kit that detects phospho-serine, -tyrosine and -threonine residues. www.calbiochem.com/phosphodetect
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