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Position: Discussion forums > Bioscience biotechniques discussion forums > Immunology forum and cytometry forum
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Antibody His-Tag
Posted by: cdrouin (IP Hidden, New member, 1)
Date: May 6, 2005 08:40AM
Hello everyone, I did a western of a fusion protein His-Tag. There is a negativ control = no his-tag protein, but a LOT of all kind protein = looks like a ladder of all sizes.....For this one ANY protein detection = Antibody specific The partially purified his-tag fusion is detected...BUT, there is also many other proteins that I though was impurities that are also detected... They are of higher molecular weight. Any idea? Could this be multimere of my his-tag protein? Any good link that talks about his-tag protein detection? His-tag antibodies specificity? Protein interaction? TKS in advance! Christian
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