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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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transfection
Posted by: delonz (IP Hidden, New member, 1)
Date: May 24, 2005 07:39PM
I am culturing primary rat brain microvascular endothelial cells, and I want to transient transfect DNA into these cells with liposome. I am eager to learn which reagent is more suitable for my study? Thanks for your kind reply.
Re: transfection
Posted by: 5'-ATCG (IP Hidden, Unregistered user, )
Date: May 25, 2005 12:51PM
On commonly used cell lines:
lipofectamine2000 - high toxicity, good efficiency, pricy; Fugene6 - medium toxicity, good efficiency, extremely pricy; Gencarrier - low toxicity, good efficiency, not pricy; GeneJammer - medium toxicity, good efficiency, very pricy. I'll suggest to use Gencarrier-1 first to see what happens on your cells - the one with higher rate of success while at the lower cost.
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