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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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drug effect
Posted by: PFC05 (IP Hidden, New member, 1)
Date: May 24, 2005 07:34PM
Could anyone tell me, please, when we want to see the inhibitory effect of the drug on stimulated cells is it usual to add stimulation agent (LPS, for example) and inhibitory agent at the same time.
Or is it better to make preincubation with stimulator or inhibitor first? Thank you in advance!
Re: drug effect
Posted by: 5'-ATCG (IP Hidden, Unregistered user, )
Date: May 25, 2005 01:00PM
First, pre-incubate with the drug (inhibitor) first, then your stimuli.
If positive, then try co-incubation of both. If positive again, try to treat your stimuli first then the drug. Through this cycle, you will have rough idea what is your drug's action point...
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