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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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culture condition for CHO cell line
Posted by: catzzh (IP Hidden, New member, 4)
Date: April 17, 2005 02:47PM
I'm culturing CHO AuxB1 cell line for cytotoxicity assay. I used DMEM+L-glutamine+10%FCS as culture medium, but the cells didn't grow at all(didn't die). Does that mean the medium did not fit this cell line?
Re: culture condition for CHO cell line
Posted by: Anna Albert (IP Hidden, New member, 3)
Date: April 19, 2005 05:27AM
Have you tried culturing the cells in Ham's F12 (Sigma N4888) or F12K medium (plus your additives)?
Re: culture condition for CHO cell line
Posted by: 5'-ATCG (IP Hidden, Unregistered user, )
Date: April 19, 2005 08:15PM
CHO AUXB1 cells should be cultured in deficient alpha-minimal essential medium containing 10% dialyzed fetal calf serum, glycine, thymidine, and hypoxanthine.
Re: culture condition for CHO cell line
Posted by: catzzh (IP Hidden, New member, 4)
Date: April 20, 2005 02:27AM
Thank you very much! I'm using alphaMEM+ribonucleosides/deoxyribonucleosides+glutamine+10%FCS. Seems growing quite well.
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