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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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cellular culture contamination
Posted by: giu (IP Hidden, Unregistered user, )
Date: March 23, 2005 09:09AM
I work with human epithelial adherent cells and 4 days ago I Have noticed a sort of filaments attached at the surface of my cells...The same filaments also float detached from the cells... Then my cells don't grow well and at last passages a lot of them haven't adhered at the flask..I have found the same filaments in the medium that I use for my culture, I have prepared a flask with only medium (DMEM, SVF10%, Antiobiotic 1%, Non essential Aminoacide), and appearently the filaments in the medium don't grow..What do you think?
Re: cellular culture contamination
Posted by: Johnh (IP Hidden, Unregistered user, )
Date: March 23, 2005 10:34AM
hi giu,
If you see the few filaments under phase-contrast microscopy it is likely just solid fibers floating in the medium. They are most likely from fiter units. Severe mycoplasma infection will cause your cells to detach without seeing unusual stuff in the culture under microscopy. Do a PCR to determine if it is the case.
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