| |||||||||
| Login :: Register :: Search forums :: Top Users :: Reagent |
|
Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
|
Oligomycin B
Posted by: Tiffy (IP Hidden, New member, 4)
Date: April 16, 2008 12:55PM
Hello everybody,
I have a question about ATP depletion. I have seeded my tumor cell line on glas coverslips and treated them with oligomycin B (50µM, 4h). Before I have started my experiment I removed the medium with glucose and replaced it with glucose-free medium. After the incubation time and before fixation I wanted to wash my cells mit PBS. Unfortunately I washed all cells away. I could fix just a few cells. Okay, then I reduced the concentration 5µM for an hour. The same result. Who knows a concentration of oligomycin b for MCF-7? It is very urgent. I am grateful for all comments. Thanks in advance. Tiffy
|
We are moving ... Please post to our new community forums
|
|
||||||||