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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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CHO-K1 vs CHO-S cell
Posted by: VPIlabs (IP Hidden, New member, 2)
Date: April 2, 2008 07:17AM
Hi,
I have a problem concerning CHO-K1 cells. I have been trying to transfect adherent CHO-K1 cells with multiple transfection agents, which worked. After that I'd like to dissociate these transfected cells to study internalisation of the transfected receptor (encoded on the plasmid) in suspension, but I have no surviving cells after this trypsinisation. I usually trypsinize them 4 hours after transfection and grow them in fresh medium till 24h post transfection. Is there anyone who has some suggestions or who has already done this? I am able to keep non-transfected cells alive, so I think the combination of trypsinization and transfection just kills my cells. Another option is to buy CHO-S cells wich are in suspension, but I have no stirrer inside our incubators. Is it possible to grow these cells without stirring them? Or will they start to adhere? Also, do you need the serum-free medium or can you use F12 or other (cheaper) media? Thanks in advance for your reactions!
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