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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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expressing very large proteins in mammalian cells
Posted by: Bouka (IP Hidden, New member, 2)
Date: December 8, 2006 05:42AM
Hi,
Has anybody had any luck in expressing very large proteins ( over 450kDa) in mammalina cells? if yes, what cells and what evctors are most suitable? thanks B
Re: expressing very large proteins in mammalian cells
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: April 3, 2007 03:09PM
We had difficulties to express very large enzymes using different combination of e.coli strains and expression vectors. Would be interesting to know the solutions too. Thanks.
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