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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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cell synchronization
Posted by: Tiffy (IP Hidden, New member, 4)
Date: June 22, 2006 06:02AM
Hello everybody
who knows an easy aand rapid protocol to synchronize tumor cells in cell culture. I have tried with serum removal, but it didnīt work. I have starved the cells for 48 h and then I wanted to stimulate the cells with a compound. Furthermore I have the impression that my cells propagate anymore Help Thanks in advances Tiffy
Re: cell synchronization
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: June 22, 2006 10:21AM
We used to use Aphidicolin, double thymidine block, serum deprivation etc to align cells in G1 phase. Depending on which cell cycle phase you want to arrest cells, you may chose different protocols. For us the serum deprivation plus aphidicolin worked well.
Tiffy Wrote: ------------------------------------------------------- > Hello everybody > > who knows an easy aand rapid protocol to > synchronize tumor cells in cell culture. I have > tried with serum removal, but it didnīt work. I > have starved the cells for 48 h and then I wanted > to stimulate the cells with a compound. > Furthermore I have the impression that my cells > propagate anymore > Help > Thanks in advances > Tiffy
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