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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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Subcellular Fractionation
Posted by: EANON (IP Hidden, Junior member, 19)
Date: July 19, 2005 10:33AM
Does anybody know where I can get a protocol for subcellular fractionation and which kit do you think is best?
Re: Subcellular Fractionation
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: July 19, 2005 01:09PM
If you are looking for culear/cytoplasm protein fractionation the Pierce has NE-PER kit. It's simple and quick and works fine for my purpose. The disadvantage though is there is around 10% cytoplasm protein contamination in nuclear fraction and it's somewhat cell type dependent.
Re: Subcellular Fractionation
Posted by: Basshead (IP Hidden, New member, 4)
Date: February 22, 2006 06:41AM
No need for kits. Simple sucrose density gardient fractionation can often be adequate but it really depend what you want to do.
We do a lot of this sort of work. What are you trying to separtae and what degree of purity are you looking for? b
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