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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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cell transfection
Posted by: cellgun (IP Hidden, Unregistered user, )
Date: February 4, 2005 03:52PM
Who can tell me whether Effectene transfection reagent is a good kit for transfection or not?
Re: cell transfection
Posted by: LarryD (IP Hidden, Unregistered user, )
Date: February 4, 2005 03:54PM
Our experience is (1) GenCarrier, (2) lipofectamine2000, (3) Fugene-6.....based on efficiency, cost and protocol. Qiagen's always is the most expensive but not necessarily the best.
Re: cell transfection
Posted by: PhD on Trouble (IP Hidden, Unregistered user, )
Date: February 7, 2005 04:58PM
I like GenCarrier over Effectene. It is cheap, consistent, reliable and high-efficiency at least in my hands (on 293, COS-7 and primary keratinocytes).
Re: cell transfection
Posted by: hutman (IP Hidden, New member, 5)
Date: February 18, 2006 07:32AM
The best way for cell transfection is electroporation in some hypotonic buffer. The cells are not in contact with any toxic reagent, which may influence the output of your experiments. Instead, they recover very fast and start to express the protein as soon as 24 h posttransfection. We do routinely nucleofection with GTporator solution at fraction of Amaxa's price.
[www.gentrend.cz] Hutman
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