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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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Transfection of sw780 cells
Posted by: jolyann (IP Hidden, New member, 1)
Date: October 27, 2005 09:48AM
Has anyone know a transfection reagent that work on sw780 cells (urothelial cancer cell line)? I've been trying to transfect sw780 cells for a while. I tried lipofectamine 2000 and gene juice. All cells are going in suspension when transfected or I still get extremely poor transfection efficiency. I would appreciate suggestions from people who have worked with this cell line before!
Thanks,
Re: Transfection of sw780 cells
Posted by: bitha (IP Hidden, New member, 5)
Date: October 27, 2005 10:12AM
Try Fugene. it is easiest on cells, I believe.
Re: Transfection of sw780 cells
Posted by: Amy (IP Hidden, New member, 2)
Date: November 2, 2005 05:21PM
We have great success with LnCap, DU145 prostate cell line using gencarrier-1, efficiency can reach ~60%. It is the cheapest, least toxic and most effective liposome reagent in our hands compared to lipo2000, fugene-6, jetPEI, effectene or genejuice.
If gencarrier doesn't work out for you, try electroporation, nucleofector or viral vectors.
Re: Transfection of sw780 cells
Posted by: hutman (IP Hidden, New member, 5)
Date: February 18, 2006 09:08AM
I have even better results with prostate cell lines using electroporation with GTporator solution. I is basically nucleofection, expression after 24h, but more than half price of Amaxa. Check it here [www.gentrend.cz] It works for sw780, LNCaP and PC3 getting 80% efficiency.
Hutman Edited 1 times. Last edit at 02/18/06 09:10AM by hutman.
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