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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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Silicon 3t3 culture
Posted by: pedrostevo (IP Hidden, New member, 1)
Date: May 27, 2005 05:38AM
I am having trouble with some 3t3 mouse fibroblast culture. I grow them fine on treated polystyrene with DMEM (+glutamine, Foetal calf serum), but due to a proposed ellipsometer experiment I need to grow some on silicon oxide slides. I want to measure the thickness of the serum/cell induced extra cellular matrix (ECM) laid down in the culture, so don't want to add things like fibronectin etc to aid attachment to the surface as this would change the more natural ECM coating of the surface, which I am interested in.
Has anyone had experience with fibroblast on silicon? Preliminary tests show they don't seem to like it very much. Like I say I would prefer not to use a pre-coating as this would negate the point of the experiment (if I can't do it without extra coatings then I shall not bother). Is there any specific treating of the silicon surface with chemicals or methods which don't add to the surface they just make it more succeptable to DMEM culture and attachment Thanks guys, sorry its a long post! =)
Re: Silicon 3t3 culture
Posted by: 5'-ATCG (IP Hidden, Unregistered user, )
Date: May 27, 2005 11:34AM
You can tested several chemicals usually for surface treatment for proteomics (printing protein, antibody or peptide on a glass slide); or you can try some cross-linking agents.
However, you have to try which chemical works best on 3T3. Good luck.
Re: Silicon 3t3 culture
Posted by: 3'-UTR (IP Hidden, Unregistered user, )
Date: May 27, 2005 02:32PM
It has been reported that vascular smooth muscle cells adhesion well on glass on ALD alumina thin film or alumina oxide slide.
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