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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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serum and serum-free
Posted by: melisa (IP Hidden, Unregistered user, )
Date: April 29, 2005 07:44PM
i grow my cells in media with serum, and do the assays in serum free media. does it have effect on the result?
thanks
Re: serum and serum-free
Posted by: 5'-ATCG (IP Hidden, Unregistered user, )
Date: May 2, 2005 04:27PM
You should expect to have a huge difference on results between serum and serum-free condition.
That's why there is a trend to develop and to use defined, serum-free medium.......
Re: serum and serum-free
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: May 9, 2005 12:19PM
I agree with 5'ATCG. There is a significant difference between the two conditions. you could try slowly lowering the concentration of serum within your cell lines until it reaches 0%, and allow the cells to acclimiate to the no serum condition before beginning your assays. i do not know what cells you are culturing or what you use to dissociate them. based on my experience with mammalian cell lines, if you do take the cells to a no serum condition you may want to consider dissociating the cells with an alternative to tryspin which can be very rough on cells grown without serum. also serum is commonly used as a tryspin inhibitor, which would prevent your passage cells from being serum-free. try tryple express from invitrogen, a trypsin-like enzyme that is room temperature stable, more gentle to cells, and does not require a tryspin inhibitor.
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