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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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ABAE and RAW cells
Posted by: rage (IP Hidden, Unregistered user, )
Date: April 27, 2005 12:10AM
I am having trouble getting good transfection efficiency for ABAE and Raw cells. I have tried different cell densities, DNA/reagent ratio and lipofectamine 2000 & superfect with not too much luck. Have anybody have a protocol and/or reagent that works well enough to test promoter activity. If so, please let me know what is your trick.
Re: ABAE and RAW cells
Posted by: 5'-ATCG (IP Hidden, Unregistered user, )
Date: April 28, 2005 06:10PM
We have some success with GenCarrier-1 cell transfection reagent which allows us to be able to perform reporter assay on endothelial and macrophage cells. But remember to avoid serum, PBS and antibiotic during DNA/liposome formation and transfection (just follow manufacturer's instruction).
Re: ABAE and RAW cells
Posted by: rage (IP Hidden, Unregistered user, )
Date: April 29, 2005 03:12PM
Hi, 5'ATCG,
Thank you for sharing your experience. I'll try this reagent.
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