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Position: Discussion forums > Bioscience biotechniques discussion forums > Cell biology and histology methods forum
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cell separation
Posted by: cellian (IP Hidden, Unregistered user, )
Date: April 27, 2005 12:28AM
Hi,
I'm facing problems with cell separation. I am studying iron oxide (nanoparticles) uptake by hepatocytes. I was unable to separate the cells and the iron particles after the experiment, I alreday tried ficoll gradient centrifugation. Please give your valuable suggestions.
Re: cell separation
Posted by: 5'-ATCG (IP Hidden, Unregistered user, )
Date: April 28, 2005 06:19PM
The easiest way is to "filter" the mixture through different pore-size membranes based on your cell size and nanoparticle (8-20 micro vs. submicro???).
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