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HisTag Protein purification WITHOUT imidazol
Posted by: Eva (IP Hidden, New member, 2)
Date: May 3, 2005 09:55AM
Hi everybody!
I have a big problem. I want to purify a his-tag protein out of saccharomyces cerevisiae cells. It is a recombinant protein that normaly exists in maize ovules an influences the growth of pollentubes. This is the thing that I want to test: How do pollentubes react in presence of this protein. All methods that I found contain imidazol in the buffers for purification. But this is toxic to pollentubes, so that my experiment would not work. Does anyone of you know a method without imidazol? Thanks, EVA
Re: HisTag Protein purification WITHOUT imidazol
Posted by: bassamfahmawi (IP Hidden, Regular member, 42)
Date: May 3, 2005 11:18AM
Re: HisTag Protein purification WITHOUT imidazol
Posted by: Eva (IP Hidden, New member, 2)
Date: May 4, 2005 04:34AM
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