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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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HisTag Protein purification WITHOUT imidazol
Posted by: Eva (IP Hidden, New member, 2)
Date: May 3, 2005 06:55AM
Hi everybody!
I have a big problem. I want to purify a his-tag protein out of saccharomyces cerevisiae cells. It is a recombinant protein that normaly exists in maize ovules an influences the growth of pollentubes. This is the thing that I want to test: How do pollentubes react in presence of this protein. All methods that I found contain imidazol in the buffers for purification. But this is toxic to pollentubes, so that my experiment would not work. Does anyone of you know a method without imidazol? Thanks, EVA
Re: HisTag Protein purification WITHOUT imidazol
Posted by: bassamfahmawi (IP Hidden, Regular member, 42)
Date: May 3, 2005 08:18AM
I am not aware of any method other than imidazole, however, you can alwways exchange it with PBS by dialysis, and thus may be you need to do this as an altrantive. good Luck!
Re: HisTag Protein purification WITHOUT imidazol
Posted by: Eva (IP Hidden, New member, 2)
Date: May 4, 2005 01:34AM
HI!
Thanks for your Answer! A dialyses is also wath I already thought about.....
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