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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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silver staining : black and brown spots
Posted by: Froquet (IP Hidden, New member, 2)
Date: April 19, 2005 04:27AM
Hi everybody, After silver staining of my 2D SDS-Page gels (10% acrylamide), some of the spots are dark/grey and some other are yellow/brown. does anyone know why? thanks a lot. Froquet
Re: silver staining : black and brown spots
Posted by: bassamfahmawi (IP Hidden, Regular member, 42)
Date: May 4, 2005 08:02AM
Different proteins stain produce different colors when stained with Silver staining, although no one to my knowledge has provided the reasoning for this, this stain is not quantitative (some attempts have been done to do so?)and thus the darker the spot or band is does not mean that there is more protein or an indication of high concentration. Some proteins color yellow, orange, brown, dark brown etc…
I hope this information is of help to you, good Luck!
Re: silver staining : black and brown spots
Posted by: sivakmar (IP Hidden, Unregistered user, )
Date: July 17, 2005 06:47AM
henever i do the silver staining, I get the dark blackish brown patches along the edges of the gels. any suggestion to avoid this problem to make the gel perfect
Re: silver staining : black and brown spots
Posted by: shane (IP Hidden, Unregistered user, )
Date: July 31, 2005 03:35AM
boss greying of gel is due to agno3 , wash ur gel proreply before adding sodium carbonate and just stop the rxn when u see ur bands appearing, this should be quick
Re: silver staining : black and brown spots
Posted by: brandiy (IP Hidden, New member, 1)
Date: September 8, 2005 06:18AM
Hi,
could it be that you´re dealing also with some metalo-Proteins and the silver is not able to stain them of course of the other metall´s around, think I read something in that direction... but I´m really not sure about that. Good luck Brandiy
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