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Quantifying Western Blots
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: March 1, 2005 04:13PM
In the past, I have used a CCD camera set-up to obtain western blot images and then processed and quantified the data (IDV) using Scion Image. However the CCD system is not currently available to our lab. I know the systems can be quite expensive, so I need another way to capture the image for data analysis. Is simply scanning the image okay? Or do I need to use a camera? I would be grateful for any help. Thanks! -pat
Re: Quantifying Western Blots
Posted by: huangz (IP Hidden, Unregistered user, )
Date: March 1, 2005 04:35PM
If you want to quantify the bands the scanning densitometer is most suitable for the purpose. The linear scanner is less precise and isn't suitable for quantification, in my opionion.
Re: Quantifying Western Blots
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: March 2, 2005 03:51PM
Huangz,
Thanks. Just what I thought. Now to explain that to the PI.
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