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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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2D SDS PAGE
Posted by: bluedragon (IP Hidden, New member, 1)
Date: February 6, 2005 07:06PM
i am using bio-rad sequential extraction kit for extracting proteins from Moraxella catarrhalis for 2D SDS PAGE. It has 3 reagents for extraction of proteins depending on its solubility. I did get proetin spots in the first two gels run by first and second extraction reagents in the kit! Unfortunately, i did not get any spots for the third one!! Can u suggest of something !!
Re: 2D SDS PAGE
Posted by: Ricardo (IP Hidden, Unregistered user, )
Date: February 24, 2005 03:01PM
Strong horizontal stripes across the gel is almost reported as impurities in agarose overlay or equilibration solution. What other possible cause could be showing this symptom
Re: 2D SDS PAGE
Posted by: Ricardo (IP Hidden, Unregistered user, )
Date: February 24, 2005 03:02PM
Strong horizontal stripes across the gel is almost reported as impurities in agarose overlay or equilibration solution. What other possible cause could be showing this symptom
Re: 2D SDS PAGE
Posted by: M.Nickal Johnson (IP Hidden, Unregistered user, )
Date: February 28, 2005 06:24AM
I want to know about the 2D SDS PAGE in detail Thanking you
Re: 2D SDS PAGE
Posted by: bassamfahmawi (IP Hidden, Regular member, 42)
Date: March 14, 2005 01:46PM
It seems that you need to desalt your protein sample using desalting spin columns befor your run your sample on 2D gele electrophoresis. Check www.piercenet.com for 2d procedures as well.
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