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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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protein purification from acrylamide gel
Posted by: monsa (IP Hidden, New member, 9)
Date: January 26, 2005 05:35PM
Hi, I need to purify a protein band from a SDS-PAGE and then elute it to run a Western blot. Does anybody have a protocol to extract proteins from SDS-PAGE?
Thanks Monsa
Re: protein purification from acrylamide gel
Posted by: huangz123 (IP Hidden, Junior member, 19)
Date: January 26, 2005 09:54PM
Protocol 1: [biowww.net]
Protocol 2: [biowww.net] I am curiouse why you want to gel purify your band and run another electrophoresis and western
Re: protein purification from acrylamide gel
Posted by: monsa (IP Hidden, New member, 9)
Date: January 27, 2005 11:43AM
huangz123: Thanks for the protocols .
I want to recover a protein band from a SDS-PAGE and then use the eluted sample for a Western Blot. I want to know that I cut the right part of the gel before sending it for Mass Spec identification. Monsa
Re: protein purification from acrylamide gel
Posted by: mamuller (IP Hidden, Junior member, 10)
Date: January 31, 2005 05:42AM
A good protocol, cut the gel fragment of interest, electro elut by inserting gel into a GeBAflex-tube & buffer, see protocol details at www.geba.org Go to protein polyacrylamide gel extraction, at bottom of page you will find full detailed protocol,
Contact me if further help is required Maurice
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