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    Ratio of stacking and separating gel
    Posted by: Tiffy (IP Hidden, New member, 4)
    Date: April 25, 2008 01:10PM

    Hello everybody,
    My problem is that I have a large volume of a protein sample. But my capacity of the gel pockets are restricted. Now my question can I pour a gel with a long stacking gel that my volume will fit properly? Because I don`t want to precipitate my samples. Or will I get problems? I am not sure. Once I read one should have a long separating gel. My mw of my protein is about 180kDa.
    Please help me.
    Thanks
    Tiffy

     

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