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Intact epitopes in Western Blot
Posted by: Inja (IP Hidden, New member, 1)
Date: March 16, 2008 03:19AM

Something I can't quite grasp,

How is it one can detect proteins in Western blot using antibodies,
if the epitopes recognised by these antibodies have been denatured
in the SDS-PAGE run beforehand?

Are you just hoping that the epitopes are continuous or that they
partially reform upon transfer to the membrane?

Thanks

 

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