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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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Can CIAP treatment increase protein MW in western blot?
Posted by: phospho (IP Hidden, New member, 1)
Date: March 9, 2008 11:10PM
I used CIAP treatment for cell lysate and IP sample. Both of them showed my target protein band shift to upper position in western blot analysis. Is that really a dephosphorylation? How can the protein become bigger after dephosphorylation? Thanks!
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