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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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protein mass spec question
Posted by: ecolinat (IP Hidden, New member, 1)
Date: February 21, 2008 04:51PM
Hi,
I recently sent a gel plug of purified BSA (as a test) to be analysed by MOLD-TOFTOF and I ran the resulting peptide masses thru Mascot and Protein Prosepector. Mascot gave me a 100% CI that it was BSA-precurser, while PP returned a high Mowse score even though the peptide coverage was only 50% (only half the peptides matched). How can that be? I am fairly new to MS and would really appreciate the help. Thank you so much Nataly Zeitouni CSULB Microbiology
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