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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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chIP problem
Posted by: mb80 (IP Hidden, Junior member, 12)
Date: January 30, 2008 09:06PM
Hi,
I am having trouble with my chIP protocol. It worked twice in December, when I was able to get fold enrichments for my protein of interest of roughly 5.0 relative to IgG, and for Pol2 of 5.0-20.0, at my promoter of interest. In Jan, those numbers went down to <2.0 If anyone can give me input on how my sonications look, I would very much appreciate it--- "A" and "B" worked, "C" and "D" did NOT work well Do you think the relatively subtle differences between A/B and C/D could account for my troubles? Thanks for input!
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