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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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IPTG induction
Posted by: wghamdi (IP Hidden, New member, 3)
Date: August 22, 2007 01:38PM
Could any body tell me why IPTG induction should be done at 20 C. Does it make any diiference if the cells were induced at higher tempreture
Thanks
Re: IPTG induction
Posted by: subaru_guin (IP Hidden, New member, 3)
Date: September 15, 2007 03:44AM
Our lab constantly test IPTG induction under different temperatures because there is an optimum temperature for max expression for each protein. We've tested a range from 4oC to 37oC.
Re: IPTG induction
Posted by: jaya gosula (IP Hidden, New member, 3)
Date: February 20, 2008 04:56AM
I have cloned the gene of 1.2 Kb into pET vector. when inducing with IPTG or lactose i was not getting any expression.
i tried lower temperatures, i tried diffrent IPTG and LActose concentrations. same gene was expressing under pBAD promoter? can u suggest me what to do to get expression under T7 promoter?
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