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PAGE on hydrophobic proteins
Posted by: manojc (IP Hidden, New member, 1)
Date: April 14, 2006 12:19PM

Hi,

I am trying to run SDS PAGE on some artificial proteins synthesized in the lab. These proteins are very hydrophobic, so when I load them in the SDS gels, all I see is dark band in the wells after staining. I also performed western (these contain 10X His Tag) and see the same...for some reason these proteins are not flowing through the gels and are precipitating in the wells. I dialyze these proteins to remove Urea and freeze dry, the protein becomes completely insoluble so I am not able to characterize them either with some other techniques.

Is there a way that I can characterize these proteins or to see them on gels? Your help will really be appreciated.

Thanks,
Manoj

 

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Re: PAGE on hydrophobic proteins
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: April 15, 2006 10:24AM

You can try tricine gel system (H. Schagger and G. von Jagow). It gives good resolution for hydrophobic proteins.

[www-bsac.eecs.berkeley.edu]

 

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