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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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2' Ab background
Posted by: justme (IP Hidden, New member, 1)
Date: April 5, 2006 10:07PM
Hello Everyone,
All I need is just a clear pics for the paper. I'm trying to confirm that the cell line is what it is, and that's it. I'm probing my total protein nitrocellulose membrane with the cell surface markers, and I got bands I wanted, but... how can I rid of the 2'Ab background? I tried three different 2'Ab brands and still have them bound to almost every single protein onto membrane! Without primary Abs, of course. Well, except markers bands though... Agh! Anyone?
Re: 2' Ab background
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: June 23, 2006 10:35AM
you may be using too much 2'Abs. try diluting it at least 10 fold.
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