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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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How to make the soluble protein from the insoluble protein
Posted by: Chien (IP Hidden, New member, 2)
Date: March 29, 2006 01:49AM
Hi all members
My name is Pham Ngoc Chien, I am studying in protein. I am purifying the protein from Bacillus anthracis now , but it is not lucky, my protein is the most insoluble protein. I have changed the IPTG concentration and induction time, I have tried to change many condition of induction temperature and induction time, concentration of IPTG... but finally it is still insoluble. I hope that I will get some advice from all members. Could you show me how to make the soluble protein from the insoluble protein or how to purify the soluble protein from the insoluble protein. Thanks for your advices Pham Ngoc Chien
Re: How to make the soluble protein from the insoluble protein
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: April 5, 2006 01:36PM
you can purify insoluble inclusion body using denaturing method and try to refold them.
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