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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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membrane protein extraction for western blot
Posted by: rinatrim (IP Hidden, New member, 1)
Date: March 21, 2006 09:05AM
Hi,
I have a problem with my samples (my pellet is sticky) and I can't loud them to the gel (western blot). I got protocol but no one tried him. Maybe someone have a protocol for "membrane protein extraction"?
Re: membrane protein extraction for western blot
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: April 5, 2006 01:38PM
it seems to me you have considerable gDNA contamination. Try vortex hard or go through needles to break down DNA before loading your sample.
Re: membrane protein extraction for western blot
Posted by: rajasekhar78 (IP Hidden, New member, 1)
Date: December 27, 2007 08:53PM
hi, do you think you can send me the protocol for membrane protein extraction.
is it for plants thanks my e-mail rajasekhar78@yahoo.com
Re: membrane protein extraction for western blot
Posted by: HCscientist (IP Hidden, New member, 3)
Date: January 26, 2008 12:50AM
1) Try to sonicate samples before loading.
2) Use commericial lysis solution, such as CellLytic-M (sigma)
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