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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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Protein G, Non specific kinase binding.
Posted by: ianbirmingham (IP Hidden, New member, 7)
Date: March 13, 2006 06:53AM
Hi, I have been doing some IP's to do some invitro kinase reactions, problem is I have stumbled upon a kinase that is binding non-specifically to protein G, which is phosphorylating my substrate in a highly specific manner. I would be interested to know what this kinase is. Anyone have any experience of non specific binding of kinases to Protein G. Alternatively, any ingenious ideas on how to work out what this kinase may be? Thanks for any help on this matter. Ian.
Re: Protein G, Non specific kinase binding.
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: March 19, 2006 08:00AM
I don't have such experience. You may search pubmed for protein G papers to see if it also interact with proteins other than IgG. If you are using a special organism, you may do a PSI or PHI BLAST search using the alignment of IgG binding sequence against your organism genome (assume protein G interact with the unknown kinase through the same binding domain). Then try to pick kinase candidates from results.
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