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Gel Filtration/ protein precipitation
Posted by: jbwaj (IP Hidden, New member, 3)
Date: January 17, 2006 11:27AM

Hi all
I am trying to express an inherently soluble protein in E Coli. I obtain inclusion bodies, which I solublize with urea and pass it over a gel filtration column. However the fraction which contains the most protein also contains a couple of other bacterial proteins which cause precipitation. I am using Sephadex G-75. Is there any way around it? I am not able to use the protein because its really turbid.

Thanks

B

 

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