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    optimizing protein expression in BL21(AI)-adjust arabinose % 1st or IPTG1st?
    Posted by: doremi_luv (IP Hidden, New member, 2)
    Date: April 21, 2008 02:37AM

    Hi,

    Currently i'm using the bl21(AI) cell line to express my protein. I'm still at the beginning stage whereby i optimize the % of arabinose and concentration of IPTG.

    What i'd like to know is, should i optimise the % arabinose first, and then followed by IPTG?
    or should i optimise the IPTG first, followed by % arabinose?

    THanks for any help in advance!!!

     

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