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    Intact epitopes in Western Blot
    Posted by: Inja (IP Hidden, New member, 1)
    Date: March 16, 2008 02:19AM

    Something I can't quite grasp,

    How is it one can detect proteins in Western blot using antibodies,
    if the epitopes recognised by these antibodies have been denatured
    in the SDS-PAGE run beforehand?

    Are you just hoping that the epitopes are continuous or that they
    partially reform upon transfer to the membrane?

    Thanks

     

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