| |||||||||
| Login :: Register :: Search forums :: Top Users :: Reagent |
|
Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
|
Dimer reduction / elimination
Posted by: exec (IP Hidden, New member, 3)
Date: April 17, 2007 07:01AM
hi everyone,
is there any method to eliminate the protein dimers from the recombinant protein sample. our is not a formulated sample. we are still in trial stage the sample we get after solubilization refolding purification is analysed by SDS pAGE. it does not contain preservative or surfactant. We obtain some high molecular weight bands some of which we suspect to be dimers as they bind the specific antibody. is there any reagent that can be added to our sample to get rid / eliminate protein dimers. kindly guide me on this exec
Re: Dimer reduction / elimination
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: June 27, 2007 10:39AM
Always add DTT, beta-mercaptoethanol and boil samples to disrupt disulfate bonds / protein-protein interactions...
|
We are moving ... Please post to our new community forums
|
|
||||||||