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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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small peptide expressed in mammlian cells
Posted by: ginger168 (IP Hidden, New member, 1)
Date: August 22, 2006 04:08PM
Hello,
I was wondering if somebody had some experience with expression of small peptide in mammalian cells. I wanna express a transmembrane domain in mammalian cells which is about 40aa in addition to a tag peptide. I tried two vectors( pCI-neo and pcDNA3.1) to express these recombinant peptide in mammalian cells but both of them failed. I search online and found out that other groups used in vitro translation system to express transmembrane peptide. I can not find out any information about expression of small peptide in mammalian cell. Any information you provide is much appreciated. Thanks ginger
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