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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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stripping a blot
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: June 6, 2006 04:05PM
if i allow a blot to dry, can I strip it using the glycine or tris buffer method? i seen protocols online that say the antibodies became irreversably bound after drying and others that say the membrane can be stripped once rewetted. does anyone have any personal experience?
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