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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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about glycine stripping buffer
Posted by: iwa633 (IP Hidden, New member, 1)
Date: May 4, 2006 04:22PM
can anyone tell me how glycine work in glycine stripping buffer?? thanks
Re: about glycine stripping buffer
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: June 6, 2006 03:58PM
i am not sure about the role of glycine chemically stripping the blot. i believe glycine is used because it provides a rather acidic pH, as this pH itself (and perhaps heat depending on your stripping protocol) is what dissociates the bound antibodies.
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