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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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White Blood Cell Apheresis: HELP!
Posted by: Ranx05 (IP Hidden, New member, 1)
Date: May 20, 2006 01:46AM
Any information anyone could provide regarding the following would be greatly appreciated:
I recently obtained a sample of PBMC (peripheral blood mononuclear cells) obtained by apheresis. I intend to purify a protein from this leukopheresis pack, which is only found in white blood cells. I have successfuly performed this purification several times with no incident. However, after an afternoon spent lysing red blood cells to clean up the pellet, I accidentally left the clean leukocyte pellet at 4 degrees C overnight, spun down, in PBS. I realized my error in the morning and immediately washed the pellet once more in PBS, spun down, aspirated the supernatant and froze the pellet on dry ice. My question is, is this pellet still good, or have I ruined an 800 dollar apheresis pack? Any information would be greatly appreciated. Thank you in advance!
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