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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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spacer in two-hybrid fusion
Posted by: GST (IP Hidden, New member, 2)
Date: March 31, 2006 05:59PM
Hello!
I got a problem with two-hybrid: it doesn't show interaction which surely exists. I suspect that the reason is large size of reporter protein (fused with studied protein) - it's 1067 aa residues, 170 kDa - which creates steric hindrance for interaction. So I gonna insert a spacer between reporter and studied protein to overcome this hindrance. The question is: is it a common thing to use such spacer, do people really use it (I mean I don't wonna problems with reviewers later :)) - may be somebody could give me links to publications where such things were done? And what can be used as such spacer? Thank you very much!
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