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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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How much cells needed in the in vitro assay?
Posted by: popogirl (IP Hidden, New member, 9)
Date: November 13, 2005 08:12PM
Sorry to bother everyone, I have a question about in vitro ubiquitination assays. I guess my interested protein is an ubiquitin E3 ligase. so I want to use the protein IP from the transfected cells to be subjected to in vitro ubiquitination assay. I want use the IP protein instead of recombinant protein because the former protein matain the structural formation in the cells so the result is more reliable.But the problem is I don't how much cells I need for the assays?
Would you please give me any clue? Thanks!!
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