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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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purification of myc-tagged proteins
Posted by: electrode (IP Hidden, New member, 1)
Date: October 15, 2005 12:55PM
I would like to to purify an enzyme with N-terminal c-myc tag using anti-myc agarose and elute this protein using non-denaturing conditions. Could you recommend the method, kind of anti-myc antibody conjugated to agarose (vendor), 9E10, 4A6, goat, rabbit polyclonal, etc.
Thanks!
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