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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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Regular non-specific bands in a Western
Posted by: Chenoeh (IP Hidden, New member, 5)
Date: August 18, 2005 10:12AM
I've done a couple of blots using a new antibody (anti-PEA3 from Santa Cruz). I get a strong band running at about 100kDa, which is higher then expected. However, I also get identical fainter bands running at lower weights in samples from 3 different cell lines. They don't resemble at all the bands you get from non-specific binding, and put me in mind of a protein ladder. I seem to remember from somewhere that this means something specific, but I can't remember what. Any ideas?
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