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POST IEF BAND
Posted by: shane (IP Hidden, Junior member, 10)
Date: August 9, 2005 11:42AM
i have purified a lectin , which was by affinity purification procedure, and the electroeluted sample from 10% gel when run for isoelectric focussing shows 6 distinct bands stained by comassie.
how should i interpret the result. is the protein not pure or can a protein have so many IEP. Thank u 4 ur perusal and kind suggestion, urs shane
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